rat anti lamp1 (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology
rat anti lamp1
Rat Anti Lamp1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1991 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti+lamp1/LAMP-1+Antibody/pm41783940-413-23-26
Average 96 stars, based on 1991 article reviews
Rat Anti Lamp1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1991 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rat+anti+lamp1/LAMP-1+Antibody/pm41783940-413-23-26
Average 96 stars, based on 1991 article reviews
rat anti lamp1 - by Bioz Stars,
2026-09
96/100 stars
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Labeling:Article Title: GSDME-mediated pyroptosis in microglia exacerbates demyelination and neuroinflammation in multiple sclerosis: insights from humans and cuprizone-induced demyelination model mice. Article Snippet: Demyelination, a hallmark of multiple sclerosis (MS), disrupts neural conduction due to myelin sheath degradation.. Microgliamediated inflammation plays a pivotal role in this process, with emerging evidence implicating gasdermin E (GSDME) in neuroinflammation and neurodegeneration.. However, the specific role of GSDME in MS remains unclear. Incubation:Article Title: GSDME-mediated pyroptosis in microglia exacerbates demyelination and neuroinflammation in multiple sclerosis: insights from humans and cuprizone-induced demyelination model mice. Article Snippet: Demyelination, a hallmark of multiple sclerosis (MS), disrupts neural conduction due to myelin sheath degradation.. Microgliamediated inflammation plays a pivotal role in this process, with emerging evidence implicating gasdermin E (GSDME) in neuroinflammation and neurodegeneration.. However, the specific role of GSDME in MS remains unclear. Article Title: Deficiency of galactosyl-ceramidase in adult oligodendrocytes worsens disease severity during chronic experimental allergic encephalomyelitis Article Snippet: .. For SDS-PAGE, 20 mg of proteins were loaded in NuPAGE 4–12% Bis-Tris protein gels (Invitrogen), and separated at 120V, transferred onto polyvinylidene difluoride membranes (Biorad), blocked for 1 h at room temperature with either 3% BSA or 1% Non-fat milk in TBS-tween (TBS-T), and incubated overnight at 4 C with the following primary antibodies: rabbit anti-phosphoTFEB S211 (1:1,000, S211, Cell Signaling), rabbit anti-TFEB (1:2,000 4240S, Cell Signaling), rabbit anti-phospho-AKT (1:2000, S473,Cell Signaling), mouse anti-AKT (1:3,000, 40D4 Cell Signaling), rabbit anti-LC3A/B (1:1,000, 4108 Cell Signaling), chicken antiGFAP (1:2,000, AB5541 EMD Millipore), rat anti-CD68 (1:1,000, MCA1957GA, Biorad), rabbit anti-Olig2 (1:500, in-house produced), rabbit anti-LAMP2 (1:2,000, PA1-655, Invitrogen), Article Title: Deficiency of galactosyl-ceramidase in adult oligodendrocytes worsens disease severity during chronic experimental allergic encephalomyelitis Article Snippet: For immunohistochemistry, fixed brains were cryoprotected in sucrose and optimal cutting temperature embedded before cryosectioning with a Leica CM3050 cryostat (Leica Biosystems). .. Floating sections (sagittal 30-mm cryosections) were washed with PBS three times for 10 min each, and incubated with rabbit anti-PLP (1:400, a kind gift from Robert Skoff, Wayne University), mouse anti-GALC (1:50, in-house produced monoclonal IgG), rabbit anti-PDGFR-a (1:200, SC-338, Santa Cruz Biotechnology), rat anti-PDGFR-a (1:50, Apa5, BD Pharmingen), rat anti-MBP (1:300, a kind gift from Anthony Campagnoni, UCLA), rat anti-CD68 (1:800, MCA1957GA, Biorad), mouse anti-GFAP (1:800, MAB360, EMD Millipore), rabbit anti-LAMP2 (1:300, PA1-655 Invitrogen), Produced:Article Title: Deficiency of galactosyl-ceramidase in adult oligodendrocytes worsens disease severity during chronic experimental allergic encephalomyelitis Article Snippet: .. For SDS-PAGE, 20 mg of proteins were loaded in NuPAGE 4–12% Bis-Tris protein gels (Invitrogen), and separated at 120V, transferred onto polyvinylidene difluoride membranes (Biorad), blocked for 1 h at room temperature with either 3% BSA or 1% Non-fat milk in TBS-tween (TBS-T), and incubated overnight at 4 C with the following primary antibodies: rabbit anti-phosphoTFEB S211 (1:1,000, S211, Cell Signaling), rabbit anti-TFEB (1:2,000 4240S, Cell Signaling), rabbit anti-phospho-AKT (1:2000, S473,Cell Signaling), mouse anti-AKT (1:3,000, 40D4 Cell Signaling), rabbit anti-LC3A/B (1:1,000, 4108 Cell Signaling), chicken antiGFAP (1:2,000, AB5541 EMD Millipore), rat anti-CD68 (1:1,000, MCA1957GA, Biorad), rabbit anti-Olig2 (1:500, in-house produced), rabbit anti-LAMP2 (1:2,000, PA1-655, Invitrogen), Article Title: Deficiency of galactosyl-ceramidase in adult oligodendrocytes worsens disease severity during chronic experimental allergic encephalomyelitis Article Snippet: For immunohistochemistry, fixed brains were cryoprotected in sucrose and optimal cutting temperature embedded before cryosectioning with a Leica CM3050 cryostat (Leica Biosystems). .. Floating sections (sagittal 30-mm cryosections) were washed with PBS three times for 10 min each, and incubated with rabbit anti-PLP (1:400, a kind gift from Robert Skoff, Wayne University), mouse anti-GALC (1:50, in-house produced monoclonal IgG), rabbit anti-PDGFR-a (1:200, SC-338, Santa Cruz Biotechnology), rat anti-PDGFR-a (1:50, Apa5, BD Pharmingen), rat anti-MBP (1:300, a kind gift from Anthony Campagnoni, UCLA), rat anti-CD68 (1:800, MCA1957GA, Biorad), mouse anti-GFAP (1:800, MAB360, EMD Millipore), rabbit anti-LAMP2 (1:300, PA1-655 Invitrogen), Immunofluorescence:Article Title: Evidence that mitochondria in macrophages are destroyed by microautophagy. Article Snippet: Doxycycline (1μg/ml, D9891, Sigma-Aldrich) was used for 16 h to induce E228Q-VPS4A-HA expression.Macrophageswere treatedwith a combination of LPS (100ng/ml, L2880, Sigma-Aldrich) andmouse IFNγ (10 ng/ml, 485-MI, R&D Systems). .. The following antibodies and dilutions were used for immunofluorescence (IF) staining and western blotting (WB) in this study: primary antibodies (dilution, catalog number, maker): rabbit anti-LC3 (1/500 for IF and 1/2000 forWB, PM036,MBL and 1/1000, #S9661, CST forWB), rabbit anti-p62 (1/500 for IF and 1/2000 forWB, PM045,MBL), rabbit anti-NDP52 (1/200 for IF, GTX115378, GeneTex), rabbit anti-Atg7 (1/1000 for WB, 8558S, Cell Signaling), rabbit anti-FIP200 (1/2000 for WB, 17250-1-AP, Proteintech), Article Title: Evidence that mitochondria in macrophages are destroyed by microautophagy Article Snippet: Macrophages were treated with a combination of LPS (100 ng/ml, L2880, Sigma-Aldrich) and mouse IFN-γ (10 ng/ml, 485-MI, R&D Systems). .. The following antibodies and dilutions were used for immunofluorescence (IF) staining and western blotting (WB) in this study: primary antibodies (dilution, catalog number, maker): rabbit anti-LC3 (1/500 for IF and 1/2000 for WB, PM036, MBL and 1/1000, #S9661, CST for WB), rabbit anti-p62 (1/500 for IF and 1/2000 for WB, PM045, MBL), rabbit anti-NDP52 (1/200 for IF, GTX115378, GeneTex), rabbit anti-Atg7 (1/1000 for WB, 8558S, Cell Signaling), rabbit anti-FIP200 (1/2000 for WB, 17250-1-AP, Proteintech), Staining:Article Title: Evidence that mitochondria in macrophages are destroyed by microautophagy. Article Snippet: Doxycycline (1μg/ml, D9891, Sigma-Aldrich) was used for 16 h to induce E228Q-VPS4A-HA expression.Macrophageswere treatedwith a combination of LPS (100ng/ml, L2880, Sigma-Aldrich) andmouse IFNγ (10 ng/ml, 485-MI, R&D Systems). .. The following antibodies and dilutions were used for immunofluorescence (IF) staining and western blotting (WB) in this study: primary antibodies (dilution, catalog number, maker): rabbit anti-LC3 (1/500 for IF and 1/2000 forWB, PM036,MBL and 1/1000, #S9661, CST forWB), rabbit anti-p62 (1/500 for IF and 1/2000 forWB, PM045,MBL), rabbit anti-NDP52 (1/200 for IF, GTX115378, GeneTex), rabbit anti-Atg7 (1/1000 for WB, 8558S, Cell Signaling), rabbit anti-FIP200 (1/2000 for WB, 17250-1-AP, Proteintech), Article Title: Development and characterization of a model of mucopolysaccharidosis type IVA for evaluating therapies targeting bone disease. Article Snippet: .. After blocking (40 min in PBS+10% FBS and 1 h in goat serum), cells were stained overnight at 4°C with the following antibodies: Article Title: Development and characterization of a model of mucopolysaccharidosis type IVA for evaluating therapies targeting bone disease Article Snippet: .. After blocking (40 min in PBS+10% FBS and 1 h in goat serum), cells were stained overnight at 4°C with the following antibodies: Article Title: Evidence that mitochondria in macrophages are destroyed by microautophagy Article Snippet: Macrophages were treated with a combination of LPS (100 ng/ml, L2880, Sigma-Aldrich) and mouse IFN-γ (10 ng/ml, 485-MI, R&D Systems). .. The following antibodies and dilutions were used for immunofluorescence (IF) staining and western blotting (WB) in this study: primary antibodies (dilution, catalog number, maker): rabbit anti-LC3 (1/500 for IF and 1/2000 for WB, PM036, MBL and 1/1000, #S9661, CST for WB), rabbit anti-p62 (1/500 for IF and 1/2000 for WB, PM045, MBL), rabbit anti-NDP52 (1/200 for IF, GTX115378, GeneTex), rabbit anti-Atg7 (1/1000 for WB, 8558S, Cell Signaling), rabbit anti-FIP200 (1/2000 for WB, 17250-1-AP, Proteintech), Western Blot:Article Title: Evidence that mitochondria in macrophages are destroyed by microautophagy. Article Snippet: Doxycycline (1μg/ml, D9891, Sigma-Aldrich) was used for 16 h to induce E228Q-VPS4A-HA expression.Macrophageswere treatedwith a combination of LPS (100ng/ml, L2880, Sigma-Aldrich) andmouse IFNγ (10 ng/ml, 485-MI, R&D Systems). .. The following antibodies and dilutions were used for immunofluorescence (IF) staining and western blotting (WB) in this study: primary antibodies (dilution, catalog number, maker): rabbit anti-LC3 (1/500 for IF and 1/2000 forWB, PM036,MBL and 1/1000, #S9661, CST forWB), rabbit anti-p62 (1/500 for IF and 1/2000 forWB, PM045,MBL), rabbit anti-NDP52 (1/200 for IF, GTX115378, GeneTex), rabbit anti-Atg7 (1/1000 for WB, 8558S, Cell Signaling), rabbit anti-FIP200 (1/2000 for WB, 17250-1-AP, Proteintech), Article Title: Evidence that mitochondria in macrophages are destroyed by microautophagy Article Snippet: Macrophages were treated with a combination of LPS (100 ng/ml, L2880, Sigma-Aldrich) and mouse IFN-γ (10 ng/ml, 485-MI, R&D Systems). .. The following antibodies and dilutions were used for immunofluorescence (IF) staining and western blotting (WB) in this study: primary antibodies (dilution, catalog number, maker): rabbit anti-LC3 (1/500 for IF and 1/2000 for WB, PM036, MBL and 1/1000, #S9661, CST for WB), rabbit anti-p62 (1/500 for IF and 1/2000 for WB, PM045, MBL), rabbit anti-NDP52 (1/200 for IF, GTX115378, GeneTex), rabbit anti-Atg7 (1/1000 for WB, 8558S, Cell Signaling), rabbit anti-FIP200 (1/2000 for WB, 17250-1-AP, Proteintech), Blocking Assay:Article Title: Development and characterization of a model of mucopolysaccharidosis type IVA for evaluating therapies targeting bone disease. Article Snippet: .. After blocking (40 min in PBS+10% FBS and 1 h in goat serum), cells were stained overnight at 4°C with the following antibodies: Article Title: Development and characterization of a model of mucopolysaccharidosis type IVA for evaluating therapies targeting bone disease Article Snippet: .. After blocking (40 min in PBS+10% FBS and 1 h in goat serum), cells were stained overnight at 4°C with the following antibodies: |
